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Four new mutations in the erythroid-specific 5-aminolevulinate synthase (ALAS2) gene causing X-linked sideroblastic anemia: increased pyridoxine responsiveness after removal of iron overload by phlebotomy and coinheritance of hereditary hemochromatosis.
Comparative genomes of Chlamydia pneumoniae and C. trachomatis.
Evidence for a two-base mechanism involving tyrosine-265 from arginine-219 mutants of alanine racemase.
Gene symbol: AGXT. Disease: primary hyperoxaluria type I.
4-Aminobutyrate aminotransferase (GABA-transaminase) deficiency.
Four novel mutations in the cystathionine beta-synthase gene: effect of a second linked mutation on the severity of the homocystinuric phenotype.
The opcA and (psi)opcB regions in Neisseria: genes, pseudogenes, deletions, insertion elements and DNA islands.
Two Novel Mutations in the Cystathionine beta-synthase Gene of Homocystinuric Patients.
Asp-89: a critical residue in maintaining the oligomeric structure of sheep liver cytosolic serine hydroxymethyltransferase
Tyrosine 265 of alanine racemase serves as a base abstracting alpha-hydrogen from L-alanine: the counterpart residue to lysine 39 specific to D-alanine.
Lysine-69 plays a key role in catalysis by ornithine decarboxylase through acceleration of the Schiff base formation, decarboxylation, and product release steps.
Deletion of the regulatory domain in the pyridoxal phosphate-dependent heme protein cystathionine beta-synthase alleviates the defect observed in a catalytic site mutant.
The multiple roles of conserved arginine 286 of 1-aminocyclopropane-1-carboxylate synthase. Coenzyme binding, substrate binding, and beyond.
Structure modelling and site-directed mutagenesis of the rat aromatic L-amino acid pyridoxal 5'-phosphate-dependent decarboxylase: a functional study
Structure and function of the tryptophan synthase alpha(2)beta(2) complex. Roles of beta subunit histidine 86.
Reversible dissociation/association of D-amino acid transaminase subunits. Properties of isolated active dimers and inactive monomers.
The Contribution of a Conformationally Mobile, Active Site Loop to the Reaction Catalyzed by Glutamate Semialdehyde Aminomutase
Three-dimensional structure of the Gly121Tyr dimeric form of ornithine decarboxylase from Lactobacillus 30a.
A change in reaction specificity of sheep liver serine hydroxymethyltransferase. Induction of NADH oxidation upon mutation of His230 to Tyr.
His230 of serine hydroxymethyltransferase facilitates the proton abstraction step in catalysis.
X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.
The role of residues outside the active site: structural basis for function of C191 mutants of Escherichia coli aspartate aminotransferase.
Molecular cloning of the human and murine 2-amino-3-ketobutyrate coenzyme A ligase cDNAs.
Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions.
Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491.
Citrobacter freundii tyrosine phenol-lyase: the role of asparagine 185 in modulating enzyme function through stabilization of a quinonoid intermediate.
Role of tyrosine 65 in the mechanism of serine hydroxymethyltransferase. Schirch V.
Identification of 5 novel mutations in the AGXT gene.
Functional synergism between the most common polymorphism in human alanine:glyoxylate aminotransferase and four of the most common disease-causing mutations.
Role of pro-297 in the catalytic mechanism of sheep liver serine hydroxymethyltransferase
Altering the reaction specificity of eukaryotic ornithine decarboxylase.
The role of Glu74 and Tyr82 in the reaction catalyzed by sheep liver cytosolic serine hydroxymethyltransferase.
Structural basis for the impaired channeling and allosteric inter-subunit communication in the beta A169L/beta C170W mutant of tryptophan synthase.
Gene cloning and molecular characterization of lysine decarboxylase from Selenomonas ruminantium delineate its evolutionary relationship to ornithine decarboxylases from eukaryotes.
Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Strain is more important than electrostatic interaction in controlling the pKa of the catalytic group in aspartate aminotransferase.
Site-directed mutagenesis of K396R of the 65 kDa glutamic acid decarboxylase active site obliterates enzyme activity but not antibody binding.
Role of tyrosine 114 of L-methionine gamma-lyase from Pseudomonas putida.
Recurrent mutations in P- and T-proteins of the glycine cleavage complex and a novel T-protein mutation (N145I): a strategy for the molecular investigation of patients with nonketotic hyperglycinemia (NKH).
A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluid.
The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403
Mutation of residues in the coenzyme binding pocket of Dopa decarboxylase.Effects on catalytic properties.
Beta D305A mutant of tryptophan synthase shows strongly perturbed allosteric regulation and substrate specificity.
Substrate recognition mechanism of thermophilic dual-substrate enzyme.
Glutamate 47 in 1-aminocyclopropane-1-carboxylate synthase is a major specificity determinant.
Nonketotic hyperglycinemia (glycine encephalopathy): laboratory diagnosis.
Molecular heterogeneity of myophosphorylase deficiency (McArdle's disease): a genotype-phenotype correlation study.
Modulation of the internal aldimine pK(a)'s of 1-aminocyclopropane-1-carboxylate synthase and aspartate aminotransferase by specific active site residues.
Formation in vitro of hybrid dimers of H463F and Y74F mutant Escherichia coli tryptophan indole-lyase rescues activity with L-tryptophan.
Effects of heme ligand mutations including a pathogenic variant, H65R, on the properties of human cystathionine beta-synthase.
Threonine-124 and phenylalanine-448 in Citrobacter freundii tyrosine phenol-lyase are necessary for activity with L-tyrosine.
The role of the conserved Lys68*:Glu265 intersubunit salt bridge in aspartate aminotransferase kinetics: multiple forced covariant amino acid substitutions in natural variants.
Two new mutations in the myophosphorylase gene in Italian patients with McArdle's disease.
Mutation of tyrosine 332 to phenylalanine converts dopa decarboxylase into a decarboxylation-dependent oxidative deaminase.
Alleviation of intrasteric inhibition by the pathogenic activation domain mutation, D444N, in human cystathionine beta-synthase.
The dual-specific active site of 7,8-diaminopelargonic acid synthase and the effect of the R391A mutation.
Deletion mutagenesis of human cystathionine beta-synthase. Impact on activity, oligomeric status, and S-adenosylmethionine regulation.
Contribution of Lys276 to the conformational flexibility of the active site of glutamate decarboxylase from Escherichia coli.
Identification of amino acid residues, essential for maintaining the tetrameric structure of sheep liver cytosolic serine hydroxymethyltransferase, by targeted mutagenesis.
Location of the pteroylpolyglutamate-binding site on rabbit cytosolic serine hydroxymethyltransferase.
On the role of alphaThr183 in the allosteric regulation and catalytic mechanism of tryptophan synthase.
Conformational change in aspartate aminotransferase on substrate binding induces strain in the catalytic group and enhances catalysis.
Characterization of the S272A,D site-directed mutations of O-acetylserine sulfhydrylase: involvement of the pyridine ring in the alpha,beta-elimination reaction.
Ornithine decarboxylase promotes catalysis by binding the carboxylate in a buried pocket containing phenylalanine 397.
Structure-function relationship in serine hydroxymethyltransferase.
Alpha,beta-elimination reaction of O-acetylserine sulfhydrylase. Is the pyridine ring required?
The molecular pathway for the allosteric regulation of tryptophan synthase.
Characterization of histidinol phosphate aminotransferase from Escherichia coli.
Role of proline residues in the folding of serine hydroxymethyltransferase.
Surface-exposed tryptophan residues are essential for O-acetylserine sulfhydrylase structure, function, and stability.
The molecular basis of glutamate formiminotransferase deficiency.
2H, 13C, and 15N kinetic isotope effects on the reaction of the ammonia-rescued K258A mutant of aspartate aminotransferase.
Reaction intermediate structures of 1-aminocyclopropane-1-carboxylate deaminase: insight into PLP-dependent cyclopropane ring-opening reaction.
Lysine 238 is an essential residue for alpha,beta-elimination catalyzed by Treponema denticola cystalysin.
Conversion of a PLP-Dependent Racemase into an Aldolase by a Single Active Site Mutation.
A serine-to-phenylalanine substitution leads to loss of alanine:glyoxylate aminotransferase catalytic activity and immunoreactivity in a patient with primary hyperoxaluria type 1.
Genetic and biochemical characterization of the trpB8 mutation of Escherichia coli tryptophan synthase. An amino acid switch at the sharp turn of the trypsin-sensitive "hinge" region diminishes substrate binding and alters solubility.
Substitution of glutamic acid 109 by aspartic acid alters the substrate specificity and catalytic activity of the beta-subunit in the tryptophan synthase bienzyme complex from Salmonella typhimurium.
A glycine-to-glutamate substitution abolishes alanine:glyoxylate aminotransferase catalytic activity in a subset of patients with primary hyperoxaluria type 1.
Point mutations in the tyrosine aminotransferase gene in tyrosinemia type II.
Cloning and expression of the Erwinia herbicola tyrosine phenol-lyase gene in Escherichia coli.
Dominant negative mutants of ornithine decarboxylase.
Partial reactions of bacterial D-amino acid transaminase with asparagine substituted for the lysine that binds coenzyme pyridoxal 5'-phosphate.
Role of aspartate-133 and histidine-458 in the mechanism of tryptophan indole-lyase from Proteus vulgaris.
The critical structural role of a highly conserved histidine residue in group IIamino acid decarboxylases.
Three-dimensional structure of kynureninase from Pseudomonas fluorescens.
Conserved and nonconserved residues in the substrate binding site of 7,8-diaminopelargonic acid synthase from Escherichia coli are essential for catalysis.
Heterodimeric interactions among the 1-amino-cyclopropane-1-carboxylate synthase polypeptides encoded by the Arabidopsis gene family.
Conformational changes and subunit communication in tryptophan synthase: effect of substrates and substrate analogs.
Pyridoxal 5'-phosphate dependent histidine decarboxylase. Overproduction, purification, biosynthesis of soluble site-directed mutant proteins, and replacement of conserved residues.
Serine Hydroxymethyltransferase: Role of Glu75 and Evidence that Serine Is Cleaved by a Retroaldol Mechanism.
Escherichia coli serine hydroxymethyltransferase. The role of histidine 228 in determining reaction specificity.
Visualization of PLP-bound intermediates in hemeless variants of human cystathionine beta-synthase: evidence that lysine 119 is a general base.
Site-directed mutagenesis provides insight into racemization and transamination of alanine catalyzed by treponema denticola cystalysin.
Molecular cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and serotonin biosynthesis.
Structural Analysis of Pseudomonas 1-Aminocyclopropane-1-carboxylate Deaminase Complexes: Insight into the Mechanism of a Unique Pyridoxal-5'-phosphate Dependent Cyclopropane Ring-Opening Reaction(,).
Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.
Crystallization and preliminary X-ray analysis of the apo form of Escherichia coli tryptophanase.
The role of Lys272 in the pyridoxal 5-phosphate active site of Synechococcus glutamate-1-semialdehyde aminotransferase.
Role of Asp222 in the catalytic mechanism of Escherichia coli aspartate aminotransferase: the amino acid residue which enhances the function of the enzyme-bound coenzyme pyridoxal 5'-phosphate.
Strand-separating conformational polymorphism analysis: efficacy of detection of point mutations in the human ornithine delta-aminotransferase gene.
Evidence that mutations in a loop region of the alpha-subunit inhibit the transition from an open to a closed conformation in the tryptophan synthase bienzyme complex.
Identification of a common mutation in Finnish patients with nonketotic hyperglycinemia.
Activity and spectroscopic properties of the Escherichia coli glutamate 1-semialdehyde aminotransferase and the putative active site mutant K265R.
Site-directed mutagenesis of the beta subunit of tryptophan synthase from Salmonella typhimurium. Role of active site glutamic acid 350.
Identification of mutations associated with peroxisome-to-mitochondrion mistargeting of alanine/glyoxylate aminotransferase in primary hyperoxaluria type 1.
Serine hydroxymethyltransferase: origin of substrate specificity.
The K258R mutant of aspartate aminotransferase stabilizes the quinonoid intermediate.
Site-directed mutagenesis of Escherichia coli aspartate aminotransferase: role of Tyr70 in the catalytic processes.
Tyr225 in aspartate aminotransferase: contribution of the hydrogen bond between Tyr225 and coenzyme to the catalytic reaction.
Effect of substitution of a lysyl residue that binds pyridoxal phosphate in thermostable D-amino acid aminotransferase by arginine and alanine.
Mechanism of mutual activation of the tryptophan synthase alpha and beta subunits. Analysis of the reaction specificity and substrate-induced inactivation of active site and tunnel mutants of the beta subunit.
The tyrosine-225 to phenylalanine mutation of Escherichia coli aspartate aminotransferase results in an alkaline transition in the spectrophotometric and kinetic pKa values and reduced values of both kcat and Km.
Activity and structure of the active-site mutants R386Y and R386F of Escherichia coli aspartate aminotransferase.
Structural and expression analyses of normal and mutant mRNA encoding glycine decarboxylase: three-base deletion in mRNA causes nonketotic hyperglycinemia.
Structural and functional role of the amino-terminal region of porcine cytosolic aspartate aminotransferase. Catalytic and structural properties of enzyme derivatives truncated on the amino-terminal side.
The role of His143 in the catalytic mechanism of Escherichia coli aspartate aminotransferase.
Autonomous folding and coenzyme binding of the excised pyridoxal 5'-phosphate binding domain of aspartate aminotransferase from Escherichia coli.
Pyridoxal phosphate-dependent histidine decarboxylases. Cloning, sequencing, and expression of genes from Klebsiella planticola and Enterobacter aerogenes and properties of the overexpressed enzymes.
Primary hyperoxaluria type I due to a point mutation of T to C in the coding region of the serine:pyruvate aminotransferase gene.
Aspartate aminotransferase with the pyridoxal-5'-phosphate-binding lysine residue replaced by histidine retains partial catalytic competence.
Substitution of glutamine for lysine at the pyridoxal phosphate binding site of bacterial D-amino acid transaminase. Effects of exogenous amines on the slow formation of intermediates.
The environments of Trp-248 and Trp-330 in tryptophan indole-lyase from Escherichia coli.
Disruption of active site interactions with pyridoxal 5'-phosphate and substrates by conservative replacements in the glycine-rich loop of Escherichia coli D-serine dehydratase.
Role of tryptophan 248 in the active site of tryptophanase from Escherichia coli.
Characterization and expression of the complementary DNA encoding rat histidine decarboxylase.
The beta subunit of tryptophan synthase. Clarification of the roles of histidine 86, lysine 87, arginine 148, cysteine 170, and cysteine 230.
Site-directed mutagenesis of the cysteinyl residues and the active-site serine residue of bacterial D-amino acid transaminase.
[Arg292----Val] or [Arg292----Leu] mutation enhances the reactivity of Escherichia coli aspartate aminotransferase with aromatic amino acids.
Contribution of a conserved arginine near the active site of Escherichia coli D-serine dehydratase to cofactor affinity and catalytic activity.
Evidence that cysteine 298 is in the active site of tryptophan indole-lyase.
Site-directed mutagenesis of Petunia hybrida 5-enolpyruvyl- shikimate-3-phosphate synthase. Lys-23 is essential for substrate binding.
The glycine-rich region of Escherichia coli D-serine dehydratase. Altered interactions with pyridoxal 5'-phosphate produced by substitution of aspartic acid for glycine.
Serine hydroxymethyltransferase: mechanism of the racemization and transamination of D- and L-alanine
Substitution of an arginyl residue for the active site lysyl residue (Lys258) of aspartate aminotransferase.
Properties of a serine hydroxymethyltransferase in which an active site histidine has been changed to an asparagine by site-directed mutagenesis.
Site-directed mutagenesis of tyrosine-71 to phenylalanine in Citrobacter freundii tyrosine phenol-lyase: evidence for dual roles of tyrosine-71 as a general acid catalyst in the reaction mechanism and in cofactor binding.
Examining the structural and chemical flexibility of the active site base, Lys-258, of Escherichia coli aspartate aminotransferase by replacement with unnatural amino acids.
Changing the reaction specificity of a pyridoxal-5'-phosphate-dependent enzyme
Role of leucine 201 of thermostable D-amino acid aminotransferase from a thermophile, Bacillus sp. YM-1.
The molecular genetic basis of myophosphorylase deficiency (McArdle's disease).
A missense mutation (I278T) in the cystathionine beta-synthase gene prevalent in pyridoxine-responsive homocystinuria and associated with mild clinical phenotype.
Two novel missense mutations in the cystathionine beta-synthase gene in homocystinuric patients.
Aminolevulinate synthase: lysine 313 is not essential for binding the pyridoxal phosphate cofactor but is essential for catalysis.
Decreasing the basicity of the active site base, Lys-258, of Escherichia coli aspartate aminotransferase by replacement with gamma-thialysine.
beta-Cystathionase from Bordetella avium. Role(s) of lysine 214 and cysteine residues in activity and cytotoxicity.
Site-directed mutagenesis of His343-->Ala in Citrobacter freundii tyrosine phenol-lyase. Effects on the kinetic mechanism and rate-determining step.
The molecular basis of homocystinuria due to cystathionine beta-synthase deficiency in Italian families, and report of four novel mutations.
Expression of apple 1-aminocyclopropane-1-carboxylate synthase in Escherichia coli: kinetic characterization of wild-type and active-site mutant forms.
Threonine dehydratases of Corynebacterium glutamicum with altered allosteric control: their generation and biochemical and structural analysis.
Structural basis for the catalytic activity of aspartate aminotransferase K258H lacking the pyridoxal 5'-phosphate-binding lysine residue.
Aminolevulinate synthase: functionally important residues at a glycine loop, a putative pyridoxal phosphate cofactor-binding site.
Characterization of a cystathionine beta-synthase allele with three mutations in cis in a patient with B6 nonresponsive homocystinuria.
Ligand-mediated changes in the tryptophan synthase indole tunnel probed by nile red fluorescence with wild type, mutant, and chemically modified enzymes.
Human cystathionine beta-synthase cDNA: sequence, alternative splicing and expression in cultured cells.
The structural basis for the altered substrate specificity of the R292D active site mutant of aspartate aminotransferase from E. coli.
The function of arginine 363 as the substrate carboxyl-binding site in Escherichia coli serine hydroxymethyltransferase.
NMR studies of 1H resonances in the 10-18-ppm range for cytosolic aspartate aminotransferase.
NMR studies of 1H resonances in the 10-18-ppm range for aspartate aminotransferase from Escherichia coli.
Identical genotypes in siblings with different homocystinuric phenotypes: identification of three mutations in cystathionine beta-synthase using an improved bacterial expression system.
Enzymological and mutational analysis of a complex primary hyperoxaluria type 1 phenotype involving alanine:glyoxylate aminotransferase peroxisome-to-mitochondrion mistargeting and intraperoxisomal aggregation.
X-linked pyridoxine-responsive sideroblastic anemia due to a Thr388-to-Ser substitution in erythroid 5-aminolevulinate synthase.
A visible marker for antisense mRNA expression in plants: inhibition of chlorophyll synthesis with a glutamate-1-semialdehyde aminotransferase antisense gene.
Shift in pH-rate profile and enhanced discrimination between dicarboxylic and aromatic substrates in mitochondrial aspartate aminotransferase Y70H.
Mechanism for the desulfurization of L-cysteine catalyzed by the nifS gene product.
Characterization of the apparent negative co-operativity induced in Escherichia coli aspartate aminotransferase by the replacement of Asp222 with alanine. Evidence for an extremely slow conformational change.
Replacement of active-site lysine-239 of thermostable aspartate aminotransferase by S-(2-aminoethyl)cysteine: properties of the mutant enzyme.
Studies of the active-site lysyl residue of thermostable aspartate aminotransferase: combination of site-directed mutagenesis and chemical modification.
Function of the active-site lysine in Escherichia coli serine hydroxymethyltransferase.
Effect of mutations at active site residues on the activity of ornithine decarboxylase and its inhibition by active site-directed irreversible inhibitors.
Gly387 of murine ornithine decarboxylase is essential for the formation of stable homodimers.
Role of an active site residue analyzed by combination of mutagenesis and coenzyme analog.
Heme biosynthesis in mammalian systems evidence of a Schiff base linkage between the pyridoxal 5'-phosphate cofactor and a lysine residue in 5-aminolevulinate synthase.
Determination of a functional lysine residue of a plant cysteine synthase bysite-directed mutagenesis, and the molecular evolutionary implications.
Structure of the complex between pyridoxal 5'-phosphate and the tyrosine 225 to phenylalanine mutant of Escherichia coli aspartate aminotransferase determined by isotope-edited classical Raman difference spectroscopy.
Role reversal for substrates and inhibitors. Slow inactivation of D-amino acid transaminase by its normal substrates and protection by inhibitors.
Molecular defect in a patient with pyridoxine-responsive homocystinuria.
Anaerobiosis and plant growth hormones induce two genes encoding 1-aminocyclopropane-1-carboxylate synthase in rice (Oryza sativa L.).
The identification of a lysine residue reactive to pyridoxal-5-phosphate in the glycerol dehydrogenase from the thermophile Bacillus stearothermophilus.
Lysine 258 in aspartate aminotransferase. Enforcer of the circe effect for amino acid substrates and general-base catalyst for the 1,3-prototropic shift.
Mutant aspartate aminotransferase (K258H) without pyridoxal-5'-phosphate-binding lysine residue. Structural and catalytic properties.
A hydrogen-bonding network modulating enzyme function: asparagine-194 and tyrosine-225 of Escherichia coli aspartate aminotransferase.
Lysine 87 in the beta subunit of tryptophan synthase that forms an internal aldimine with pyridoxal phosphate serves critical roles in transimination,catalysis, and product release.
Intersubunit location of the active site of mammalian ornithine decarboxylase as determined by hybridization of site-directed mutants.
A yeast assay for functional detection of mutations in the human cystathionine beta-synthase gene.
Catalytic ability and stability of two recombinant mutants of D-amino acid transaminase involved in coenzyme binding.
Interaction of pyridoxal 5'-phosphate with tryptophan-139 at the subunit interface of dimeric D-amino acid transaminase.
Defective cystathionine beta-synthase regulation by S-adenosylmethionine in a partially pyridoxine responsive homocystinuria patient.
The mechanism of high-yielding chiral syntheses catalysed by wild-type and mutant forms of aspartate aminotransferase.
Homocysteine response to methionine challenge in four obligate heterozygotes for homocystinuria and relationship with cystathionine beta-synthase mutations.
Cloning of bovine muscle glycogen phosphorylase cDNA and identification of a mutation in cattle with myophosphorylase deficiency, an animal model for McArdle's disease.
Site-directed mutagenesis techniques in the study of Escherichia coli serine hydroxymethyltransferase.
A change in the internal aldimine lysine (K42) in O-acetylserine sulfhydrylase to alanine indicates its importance in transimination and as a general base catalyst.
Functionally important residues of aromatic L-amino acid decarboxylase probed by sequence alignment and site-directed mutagenesis.
Structural studies on folding intermediates of serine hydroxymethyltransferase using fluorescence resonance energy transfer.
Mutations at a glycine loop in aminolevulinate synthase affect pyridoxal phosphate cofactor binding and catalysis.
Site-directed mutagenesis of the amino acid residues in beta-strand III [Val30-Val36] of D-amino acid aminotransferase of Bacillus sp. YM-1.
Two novel mutations (K384E and L539S) in the C-terminal moiety of the cystathionine beta-synthase protein in two French pyridoxine-responsive homocystinuria patients.
Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana.
Substitutions of alanine for cysteine at a reactive thiol site and for lysine at a pyridoxal phosphate binding site of 1-aminocyclopropane-1-carboxylate deaminase.
Role of Arg-277 in the binding of pyridoxal 5'-phosphate to Trypanosoma brucei ornithine decarboxylase.
An anomalous side reaction of the Lys303 mutant aromatic L-amino acid decarboxylase unravels the role of the residue in catalysis.
Characterisation of five missense mutations in the cystathionine beta-synthase gene from three patients with B6-nonresponsive homocystinuria.
L-allo-threonine aldolase from Aeromonas jandaei DK-39: gene cloning, nucleotide sequencing, and identification of the pyridoxal 5'-phosphate-binding lysine residue by site-directed mutagenesis.
The crystal structure of Citrobacter freundii tyrosine phenol-lyase complexed with 3-(4'-hydroxyphenyl)propionic acid, together with site-directed mutagenesis and kinetic analysis, demonstrates that arginine 381 is required for substrate specificity.
Primary hyperoxaluria type 1: a cluster of new mutations in exon 7 of the AGXT gene.
Importance of the amino terminus in maintenance of oligomeric structure of sheep liver cytosolic serine hydroxymethyltransferase.
Analysis of CBS alleles in Czech and Slovak patients with homocystinuria: report on three novel mutations E176K, W409X and 1223 + 37 del99.
Active-site Arg --> Lys substitutions alter reaction and substrate specificity of aspartate aminotransferase.
Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities. Gene cloning, purification, and characterization of a novel pyridoxal enzyme.
Mutation of cysteine 111 in Dopa decarboxylase leads to active site perturbation.
The role of His-134, -147, and -150 residues in subunit assembly, cofactor binding, and catalysis of sheep liver cytosolic serine hydroxymethyltransferase.
Functional modeling of vitamin responsiveness in yeast: a common pyridoxine-responsive cystathionine beta-synthase mutation in homocystinuria.
Gene cloning, nucleotide sequencing, and purification and characterization of the low-specificity L-threonine aldolase from Pseudomonas sp. strain NCIMB 10558.
Role of arginine 439 in substrate binding of 5-aminolevulinate synthase.
Mutation analysis in myophosphorylase deficiency (McArdle's disease).
Aspartate-279 in aminolevulinate synthase affects enzyme catalysis through enhancing the function of the pyridoxal 5'-phosphate cofactor.
Mutations in the liver glycogen phosphorylase gene (PYGL) underlying glycogenosis type VI.
The SPL1 tRNA splicing gene of Candida maltosa and Candida albicans.
Mutation of an active site residue of tryptophan synthase (beta-serine 377) alters cofactor chemistry.
Complementation analysis of mutants of 1-aminocyclopropane- 1-carboxylate synthase reveals the enzyme is a dimer with shared active sites.
Directed evolution of an aspartate aminotransferase with new substrate specificities.
Role of Arg-401 of cytosolic serine hydroxymethyltransferase in subunit assembly and interaction with the substrate carboxy group.
The role of lysine-256 in the structure and function of sheep liver recombinant serine hydroxymethyltransferase.
The role of tyrosine 121 in cofactor binding of 5-aminolevulinate synthase.
Bacterial selenocysteine synthase--structural and functional properties.
Cysteine 42 is important for maintaining an integral active site for O-acetylserine sulfhydrylase resulting in the stabilization of the alpha-aminoacrylate intermediate.
Cryocrystallography and microspectrophotometry of a mutant (alpha D60N) tryptophan synthase alpha 2 beta 2 complex reveals allosteric roles of alpha Asp60.
Mutation of aspartate-233 to valine in mouse ornithine decarboxylase reduces enzyme activity.
Pyridoxal phosphate binding to wild type, W330F, and C298S mutants of Escherichia coli apotryptophanase: unraveling the cold inactivation.
Crystal structures of L201A mutant of D-amino acid aminotransferase at 2.0 A resolution: implication of the structural role of Leu201 in transamination.
The structure of serine hydroxymethyltransferase as modeled by homology and validated by site-directed mutagenesis.
Tryptophan synthase mutations that alter cofactor chemistry lead to mechanism-based inactivation.
The novel substrate recognition mechanism utilized by aspartate aminotransferase of the extreme thermophile Thermus thermophilus HB8.
Transamination as a side-reaction catalyzed by alanine racemase of Bacillus stearothermophilus.
Isolation and characterization of ACC deaminase genes from two different plant growth-promoting rhizobacteria.
Conversion of tyrosine phenol-lyase to dicarboxylic amino acid beta-lyase, an enzyme not found in nature.
Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues.
Functional and structural analysis of cis-proline mutants of Escherichia coli aspartate aminotransferase.
Effects of the E177K mutation in D-amino acid transaminase. Studies on an essential coenzyme anchoring group that contributes to stereochemical fidelity.
Role of lysine 39 of alanine racemase from Bacillus stearothermophilus that binds pyridoxal 5'-phosphate. Chemical rescue studies of Lys39 --> Ala mutant.

 

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