PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 98
PROTEIN NAME: Serine hydroxymethyltransferase, cytosolic (EC 2.1.2.1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT).
SWISS PROT ENTRY: GLYC_SHEEP
ORGANISM: OVIS ARIES SHEEP
GENE NAME: SHMT1
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A970196

MUTATIONS:
DELETION Ala--Lys 1-6

EFFECTS: Secondary structure [=] (by CD spectrum): Oligometric status of holoenzyme [=] (tetramer): Oligometric status of apoenzyme [=] (a mixture of teramers and dimers): Reconstitution to active tetrameric species by addition of pyridoxal-P to apoenzyme [=] FUNCTION Specific activity [=]: Km for serine [=] and 5,6,7,8-tetra- hydrofolate (H4-folate) [=] STABILITY Thermal stability [=]: Stability against urea [=]

REFERENCE:
PUBMED ID: 9249049
TITLE:Importance of the amino terminus in maintenance of oligomeric structure of sheep liver cytosolic serine hydroxymethyltransferase.
AUTHOR:Jagath JR, Sharma B, Bhaskar B, Datta A, Rao NA, Savithri HS.
REFERENCE:Eur J Biochem. 1997 Jul 1;247(1):372-9.

3D STRUCTURE: Null