MUTANT ACCESSION NUMBER: 97
PROTEIN NAME: Serine hydroxymethyltransferase, cytosolic (EC 2.1.2.1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT).
SWISS PROT ENTRY:
GLYC_SHEEP
ORGANISM: OVIS ARIES SHEEP
GENE NAME: SHMT1
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A971053
MUTATIONS:
POINT Arg 401 Ala
EFFECTS: Secondary structure [=] (by far-UV CD): Oligomeric status
of holoenzyme [=] (homotetramer): Oligomeric status of
apoenzyme is mostly in the tetramer (> 90%).(wild-type
apoenzyme eluted as a mixture of tetramer (60%) and dimer
(40%))
STABILITY Apoenzyme stored at 4 C for 48h is present as a tetramer and on
addition of PLP, it could be reconstituted to a tetramer
almost completely.: Thermal stability [+] in the absence of
ligands (defined as Tm): The addition of ligands (L-serine, L-
serinamide, glycine and D-alanine) do not change Tm.
FUNCTION Specific activity [-]: Unable to catalyse the hydroxymethyl
transfer from serine: Unable to catalyse the reactions with beta-phenylserine, D-alanine or glycine.: Affected the interaction of cysteine and amino-oxyacetic acid: Not affected the interaction of methoxyamine (lacks the carboxy group)
REFERENCE:
PUBMED ID:
9581569
TITLE:Role of Arg-401 of cytosolic serine hydroxymethyltransferase in subunit assembly and interaction with the substrate carboxy group.
AUTHOR:Jagath JR, Rao NA, Savithri HS.
REFERENCE:Biochem J. 1997 Nov 1;327 ( Pt 3):877-82.
3D STRUCTURE: Null
© copyright 2003
|
