PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 79
PROTEIN NAME: D-alanine aminotransferase (EC 2.6.1.21) (D-aspartate aminotransferase) (D-amino acid aminotransferase) (D-amino acid transaminase) (DAAT).
SWISS PROT ENTRY: DAAA_BACSP
ORGANISM: BACILLUS SP STRAIN YM-1
GENE NAME: DAT
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A991054

MUTATIONS:
POINT Ser 180 Ala

EFFECTS: % PMP of the total cofactor content [=]Specific activity for D-Ala [=]: Km for D-Ala [=]: Km for alpha- ketoglutarate [=]: Racemase activity [+] FUNCTION Specific activity for D-Ala [=]: Km for D-Ala [=]: Km for alpha- ketoglutarate [=]: Racemase activity [+]

REFERENCE:
PUBMED ID: 9930994
TITLE:Effects of the E177K mutation in D-amino acid transaminase. Studies on an essential coenzyme anchoring group that contributes to stereochemical fidelity.
AUTHOR:van Ophem PW, Peisach D, Erickson SD, Soda K, Ringe D, Manning JM.
REFERENCE:Biochemistry. 1999 Jan 26;38(4):1323-31.

3D STRUCTURE:1DAA 2DAA 2DAB 4DAA 5DAA