PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 69
PROTEIN NAME: Alanine racemase (EC 5.1.1.1).
SWISS PROT ENTRY: ALR_BACST
ORGANISM: BACILLUS STEAROTHERMOPHILUS
GENE NAME: ALR DAL
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A990299

MUTATIONS:
POINT Lys 39 Ala

EFFECTS: Inactive but gains an activity as high as about 0.1% of that the wld-type enzyme upon addition of 0.2 M methylamine.STRUCTURE Secondary structure [=] (by far-UV and near-UV CD spectra): Aldehyde group of PLP stays in a free form in this mutant.: No CD band due to the bound PLP (The wild-type enzyme shows a negative CD band around 420nm)

REFERENCE:
PUBMED ID: 9933615
TITLE:Role of lysine 39 of alanine racemase from Bacillus stearothermophilus that binds pyridoxal 5''-phosphate. Chemical rescue studies of Lys39 --> Ala mutant.
AUTHOR:Watanabe A, Kurokawa Y, Yoshimura T, Kurihara T, Soda K, Esaki N, Watababe A.
REFERENCE:J Biol Chem. 1999 Feb 12;274(7):4189-94. Erratum in: J Biol Chem 1999 Oct 15;274(42):30334

3D STRUCTURE:1SFT 2SFP