PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 59
PROTEIN NAME: Tyrosine phenol-lyase (EC 4.1.99.2) (Beta-tyrosinase).
SWISS PROT ENTRY: TPL_CITFR
ORGANISM: CITROBACTER FREUNDII
GENE NAME: TPL
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A993914

MUTATIONS:
POINT Val 283 Arg

EFFECTS: kcat [-] (30-fold) and Km [=] for beta-elimination activity toward L-tyrosine: R100T/BV283R catalyzed beta-elimination reaction of L-aspartate, L-glutamete, and L-2-aminoadipate, while the activity toward these substrates was below detection for WT.: Activity for 3-chloro-L-alanine [-] (slightly), L-serine [0], S-methyl-L-cysteine [0], and S-(O-nitrophenyl)- L-cysteine [0]: kcat for transamination with L-aspartate [+] (10-fold), L-glutamate [+] (10-fold), L-serine [+] (2-fold), and L-alanine [+] (2-fold): kcat for racemization of L-alanine [-] (4-fold)

REFERENCE:
PUBMED ID: 9880502
TITLE:Conversion of tyrosine phenol-lyase to dicarboxylic amino acid beta-lyase, an enzyme not found in nature.
AUTHOR:Mouratou B., Kasper P., Gehring H. & Christen P.
REFERENCE:J.Biol.Chem. (1999) 274(3), 1320-1325

3D STRUCTURE:1TPL 2TPL