MUTANT ACCESSION NUMBER: 56
PROTEIN NAME: Tryptophan synthase beta chain (EC 4.2.1.20).
SWISS PROT ENTRY:
TRPB_SALTY
ORGANISM: SALMONELLA TYPHIMURIUM, AND SALMONELLA TYPHI
GENE NAME: TRPB STM1726 STY1325 T1638
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A993863
MUTATIONS:
POINT His 86 Leu
EFFECTS: Altered pH profile of absorption and fluorescence spectra of H86L alpha2beta2 complex in the absence of L-Ser: Altered pH profile of absorption and fluorescence spectra of H86L alpha2- beta2 complex in the presence of L-Ser.: Circular dichroism signal of pyridoxal phosphate (PLP) bound to alpha2beta complex [0] and of a reaction intermediate with L-Ser [0]: PLP binding of H86L alpha2beta2 complex [-] (20-fold): H86L alpha2beta2 complex altered pH profile of Vmax for the beta- elimination (L-Ser -> pyruvate + NH3) and the beta-replacement (L-Ser + indole -> L-Trp + H2O) reactions.: H86L alpha2beta2 complex shifted the pH optimum for the synthesis of L-Trp from pH 7.5 to 8.8.
REFERENCE:
PUBMED ID:
10593940
TITLE:Structure and function of the tryptophan synthase alpha(2)beta(2) complex. Roles of beta subunit histidine 86.
AUTHOR:Ro HS, Miles EW.
REFERENCE:J Biol Chem. 1999 Dec 17;274(51):36439-45
3D STRUCTURE: Null
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