PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 42
PROTEIN NAME: Aspartate aminotransferase (EC 2.6.1.1) (Transaminase A) (ASPAT).
SWISS PROT ENTRY: AAT_ECOLI
ORGANISM: ESCHERICHIA COLI
GENE NAME: ASPC B0928
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A920082

MUTATIONS:
POINT Asp 211 Glu

EFFECTS: CD spectrum of the PLP form in the region of 200-250 nm [=]: pH-dependent absorption spectral changes of the PLP form [=]FUNCTION Kd for PMP [+]: Catalytic activity in the overall-transamination reaction between aspartate and 2-oxoglutarate [=]: Catalytic efficiency in the reaction of the PLP form with aspartate [-]: Catalytic efficiency in the reaction of PMP form with 2-oxoglutarate [=]

REFERENCE:
PUBMED ID: 1610831
TITLE:Role of Asp222 in the catalytic mechanism of Escherichia coli aspartate aminotransferase: the amino acid residue which enhances the function of the enzyme-bound coenzyme pyridoxal 5''-phosphate.
AUTHOR:Yano T, Kuramitsu S, Tanase S, Morino Y, Kagamiyama H.
REFERENCE:Biochemistry. 1992 Jun 30;31(25):5878-87.

3D STRUCTURE:1AAM 1AAW 1AIA 1AIB 1AIC 1AMQ 1AMR 1AMS 1ARS 1ART 1ASA 1ASB 1ASC 1ASD 1ASE 1ASF 1ASG 1ASL 1ASM 1ASN 1B4X 1BQA 1BQD 1C9C 1CQ6 1CQ7 1CQ8 1CZC 1CZE 1G4V 1G4X 1G7W 1G7X 1IX6 1IX7 1IX8 1QIR 1QIS 1QIT 1SPA 1YOO 2AAT 3AAT 5EAA