PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 38
PROTEIN NAME: D-alanine aminotransferase (EC 2.6.1.21) (D-aspartate aminotransferase) (D-amino acid aminotransferase) (D-amino acid transaminase) (DAAT).
SWISS PROT ENTRY: DAAA_BACSP
ORGANISM: BACILLUS SP STRAIN YM-1
GENE NAME: DAT
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A910517

MUTATIONS:
POINT Lys 145 Arg

EFFECTS: Specific activity under the standard assay conditions [0]:Pyridoxal phosphate bound in an active state [-]: Spectral change by addition of D-alanine [=]: Pyruvate formation [-] (slowly catalyzed half-reaction): No effect of exogenous amines on the half-reaction.

REFERENCE:
PUBMED ID: 1902115
TITLE:Effect of substitution of a lysyl residue that binds pyridoxal phosphate in thermostable D-amino acid aminotransferase by arginine and alanine.
AUTHOR:Nishimura K., Tanizawa K., Yoshimura T., Esaki N., Futaki S., Manning J.M. & Soda K.
REFERENCE:Biochemistry (1991) 30(16), 4072-4077

3D STRUCTURE:1DAA 2DAA 2DAB 4DAA 5DAA