PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 37
PROTEIN NAME: Tryptophan synthase beta chain (EC 4.2.1.20).
SWISS PROT ENTRY: TRPB_SALTY
ORGANISM: SALMONELLA TYPHIMURIUM, AND SALMONELLA TYPHI
GENE NAME: TRPB STM1726 STY1325 T1638
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A910320

MUTATIONS:
POINT Glu 350 Ala

EFFECTS: Activity of the alpha2beta2 complex in the alpha reaction [-]: Activity of the alpha2beta2 complex in the alph-beta reaction with L-Ser [- - -], beta-replacement reaction with L-Ser [0], and beta-elimination reaction with L-Ser [0]: Activity of the alpha2beta2 complex in the alph-beta reaction with beta- chloro-L-Ala [-], beta-replacement reaction beta-chloro- L-Ala [+], and beta-elimination reaction beta-chloro-L-Ala [+ +]

REFERENCE:
PUBMED ID: 1673461
TITLE:Site-directed mutagenesis of the beta subunit of tryptophan synthase from Salmonella typhimurium. Role of active site glutamic acid 350.
AUTHOR:Kayastha AM, Sawa Y, Nagata S, Miles EW.
REFERENCE:J Biol Chem. 1991 Apr 25;266(12):7618-25.

3D STRUCTURE: Null