PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 31
PROTEIN NAME: Tryptophanase (EC 4.1.99.1) (L-tryptophan indole-lyase) (TNase).
SWISS PROT ENTRY: TNAA_ECOLI
ORGANISM: ESCHERICHIA COLI, AND SHIGELLA FLEXNERI
GENE NAME: TNAA IND B3708 SF3754 S4017
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A890377

MUTATIONS:
POINT Cys 298 Ser

EFFECTS: Activity [-] with L-tryptophan and [=] with SOPC: Pyridoxal phosphate binding [-]: Shift in the cofactor absorption spectrum to 414nm.: Altered pKa.: Alkaline shift in the pH profile for catalysis.: Resistance to inactivation of the apoenzyme by bromopyruvic acid.: Isomerization to unreactive state upon storage at 4 C.

REFERENCE:
PUBMED ID: 2659590
TITLE:Evidence that cysteine 298 is in the active site of tryptophan indole-lyase.
AUTHOR:Phillips R.S. & Gollnick P.D.
REFERENCE:J.Biol.Chem. (1989) 264(18), 10627-10632

3D STRUCTURE: Null