PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 30
PROTEIN NAME: Tryptophanase (EC 4.1.99.1) (L-tryptophan indole-lyase) (TNase).
SWISS PROT ENTRY: TNAA_ECOLI
ORGANISM: ESCHERICHIA COLI, AND SHIGELLA FLEXNERI
GENE NAME: TNAA IND B3708 SF3754 S4017
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A890377

MUTATIONS:
POINT Cys 294 Ser

EFFECTS: Activity [=] with L-tryptophan and [=] with S-(o-nitrophenyl)- L-cysteine (SOPC): Pyridoxal phosphate binding [=]: Cofactor absorption spectrum [=]: pKa [=]pH profile for catalysis [=]: Rate of bromopyruvic acid inactivation [=]

REFERENCE:
PUBMED ID: 2659590
TITLE:Evidence that cysteine 298 is in the active site of tryptophan indole-lyase.
AUTHOR:Phillips R.S. & Gollnick P.D.
REFERENCE:J.Biol.Chem. (1989) 264(18), 10627-10632

3D STRUCTURE: Null