PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 16
PROTEIN NAME: Serine hydroxymethyltransferase (EC 2.1.2.1) (Serine methylase) (SHMT).
SWISS PROT ENTRY: GLYA_ECOLI
ORGANISM: ESCHERICHIA COLI, ESCHERICHIA COLI O6, AND SHIGELLA FLEXNERI
GENE NAME: GLYA B2551 C3073 SF2598 S2770
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A880266

MUTATIONS:
POINT His 228 Asn

EFFECTS: Catalytic activity [-]: Affinity for both D- and L-Ala [+]:Form quinonoid complexes with both isomers at similar rates: Transaminate both Ala isomers: Catalyze an alanine racemase reaction: Lost the ability to distinguish between D- and L-Ala.

REFERENCE:
PUBMED ID: 3069126
TITLE:Serine hydroxymethyltransferase: mechanism of the racemization and transamination of D- and L-alanine
AUTHOR:Shostak K, Schirch V.
REFERENCE:Biochemistry (1988) 27(21), 8007-8014

3D STRUCTURE:1EQB 1KL2