PLPMDB Mutant Example

MUTANT ACCESSION NUMBER: 11
PROTEIN NAME: D-alanine aminotransferase (EC 2.6.1.21) (D-aspartate aminotransferase) (D-amino acid aminotransferase) (D-amino acid transaminase) (DAAT).
SWISS PROT ENTRY: DAAA_BACSP
ORGANISM: BACILLUS SP STRAIN YM-1
GENE NAME: DAT
VARIANT OR MUTAGEN:Mutagen
PMD ENTRY: A001160

MUTATIONS:
POINT Trp 139 Phe

EFFECTS: An equilibrium between dimers and monomers is demonstrated,unlike the case of the wild-type (WT).: Pyridoxal 5''-phosphate (PLP) content of the dimer [-]: Extinction coefficient of the. Specific activity of the dimer [-] (90% of WT level):The monomers are inactive. dimer at 418 nm [-]

REFERENCE:
PUBMED ID: 10630999
TITLE:Reversible dissociation/association of D-amino acid transaminase subunits. Properties of isolated active dimers and inactive monomers.
AUTHOR:Kishimoto K., Yasuda C. & Manning J.M.
REFERENCE:Biochemistry (2000) 39(2), 381-387

3D STRUCTURE:1DAA 2DAA 2DAB 4DAA 5DAA